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5 kd cutoff filter  (Millipore)


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  • 90

    Structured Review

    Millipore 5 kd cutoff filter
    5 Kd Cutoff Filter, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5-kd+cutoff+filter/pvdf+membrane/pmc08548177-78-56-55
    Average 90 stars, based on 1 article reviews
    5 kd cutoff filter - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Synthetic peptides were incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction, which was then centrifugally filtered through a Millipore 5-kD cutoff filter.

    Article Title: Proteomic and metabolomic analyses of soybean root tips under flooding stress.
    Article Snippet: Flooding is one of the serious problems for soybean plants because it inhibits growth.. Proteomic and metabolomic techniques were used to determine whether proteins and metabolites are altered in the root tips of soybeans under flooding stress.. Two-day-old soybean plants were flooded for 2 days, and proteins and metabolites were extracted from root tips.

    Article Title: Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency
    Article Snippet: The upper layer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9100× g at 4 °C.

    Article Title: Multiple analysis of root exudates and microbiome in rice (Oryza sativa) under low P conditions.
    Article Snippet: Plants release various metabolites from roots and root exudates contribute to differences in stress tolerance among plant species.. Plant and soil microbes have complex interactions that are affected by biotic and abiotic factors.. The purpose of this study was to examine the differences in metabolites in root exudates of rice (Oryza sativa) cultivars and their correlation with bacterial populations in the rhizosphere.

    Article Title: Integrated Analysis of the Effects of Cold and Dehydration on Rice Metabolites, Phytohormones, and Gene Transcripts
    Article Snippet: Metabolites were extracted from aerial parts of rice plants (100 mg) with a chloroform:methanol solution (1:1) using a mixer and a 5-kD cutoff filter (Millipore).

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Five micrograms of each peptide (AIPLLSPLITSPTSPESDNLKA or KKWQHPASPFCYEPAP, synthesized by Ontores Biotechnology) was incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction (25 m m Tris-HCl, pH 7.5, 1 m m EGTA, 20 m m MgCl 2 , 1 m m DTT, 200 μ m ATP, and 1× phosphatase inhibitor) at room temperature for 1 h. The reaction buffer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9,000 g at 4°C.

    Liquid Chromatography:

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Synthetic peptides were incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction, which was then centrifugally filtered through a Millipore 5-kD cutoff filter.

    Article Title: Proteomic and metabolomic analyses of soybean root tips under flooding stress.
    Article Snippet: Flooding is one of the serious problems for soybean plants because it inhibits growth.. Proteomic and metabolomic techniques were used to determine whether proteins and metabolites are altered in the root tips of soybeans under flooding stress.. Two-day-old soybean plants were flooded for 2 days, and proteins and metabolites were extracted from root tips.

    Article Title: Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency
    Article Snippet: The upper layer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9100× g at 4 °C.

    Article Title: Multiple analysis of root exudates and microbiome in rice (Oryza sativa) under low P conditions.
    Article Snippet: Plants release various metabolites from roots and root exudates contribute to differences in stress tolerance among plant species.. Plant and soil microbes have complex interactions that are affected by biotic and abiotic factors.. The purpose of this study was to examine the differences in metabolites in root exudates of rice (Oryza sativa) cultivars and their correlation with bacterial populations in the rhizosphere.

    Article Title: Integrated Analysis of the Effects of Cold and Dehydration on Rice Metabolites, Phytohormones, and Gene Transcripts
    Article Snippet: Metabolites were extracted from aerial parts of rice plants (100 mg) with a chloroform:methanol solution (1:1) using a mixer and a 5-kD cutoff filter (Millipore).

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Five micrograms of each peptide (AIPLLSPLITSPTSPESDNLKA or KKWQHPASPFCYEPAP, synthesized by Ontores Biotechnology) was incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction (25 m m Tris-HCl, pH 7.5, 1 m m EGTA, 20 m m MgCl 2 , 1 m m DTT, 200 μ m ATP, and 1× phosphatase inhibitor) at room temperature for 1 h. The reaction buffer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9,000 g at 4°C.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Synthetic peptides were incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction, which was then centrifugally filtered through a Millipore 5-kD cutoff filter.

    Article Title: Proteomic and metabolomic analyses of soybean root tips under flooding stress.
    Article Snippet: Flooding is one of the serious problems for soybean plants because it inhibits growth.. Proteomic and metabolomic techniques were used to determine whether proteins and metabolites are altered in the root tips of soybeans under flooding stress.. Two-day-old soybean plants were flooded for 2 days, and proteins and metabolites were extracted from root tips.

    Article Title: Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency
    Article Snippet: The upper layer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9100× g at 4 °C.

    Article Title: Multiple analysis of root exudates and microbiome in rice (Oryza sativa) under low P conditions.
    Article Snippet: Plants release various metabolites from roots and root exudates contribute to differences in stress tolerance among plant species.. Plant and soil microbes have complex interactions that are affected by biotic and abiotic factors.. The purpose of this study was to examine the differences in metabolites in root exudates of rice (Oryza sativa) cultivars and their correlation with bacterial populations in the rhizosphere.

    Article Title: Integrated Analysis of the Effects of Cold and Dehydration on Rice Metabolites, Phytohormones, and Gene Transcripts
    Article Snippet: Metabolites were extracted from aerial parts of rice plants (100 mg) with a chloroform:methanol solution (1:1) using a mixer and a 5-kD cutoff filter (Millipore).

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Five micrograms of each peptide (AIPLLSPLITSPTSPESDNLKA or KKWQHPASPFCYEPAP, synthesized by Ontores Biotechnology) was incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction (25 m m Tris-HCl, pH 7.5, 1 m m EGTA, 20 m m MgCl 2 , 1 m m DTT, 200 μ m ATP, and 1× phosphatase inhibitor) at room temperature for 1 h. The reaction buffer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9,000 g at 4°C.

    Sequencing:

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Synthetic peptides were incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction, which was then centrifugally filtered through a Millipore 5-kD cutoff filter.

    Article Title: Proteomic and metabolomic analyses of soybean root tips under flooding stress.
    Article Snippet: Flooding is one of the serious problems for soybean plants because it inhibits growth.. Proteomic and metabolomic techniques were used to determine whether proteins and metabolites are altered in the root tips of soybeans under flooding stress.. Two-day-old soybean plants were flooded for 2 days, and proteins and metabolites were extracted from root tips.

    Article Title: Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency
    Article Snippet: The upper layer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9100× g at 4 °C.

    Article Title: Multiple analysis of root exudates and microbiome in rice (Oryza sativa) under low P conditions.
    Article Snippet: Plants release various metabolites from roots and root exudates contribute to differences in stress tolerance among plant species.. Plant and soil microbes have complex interactions that are affected by biotic and abiotic factors.. The purpose of this study was to examine the differences in metabolites in root exudates of rice (Oryza sativa) cultivars and their correlation with bacterial populations in the rhizosphere.

    Article Title: Integrated Analysis of the Effects of Cold and Dehydration on Rice Metabolites, Phytohormones, and Gene Transcripts
    Article Snippet: Metabolites were extracted from aerial parts of rice plants (100 mg) with a chloroform:methanol solution (1:1) using a mixer and a 5-kD cutoff filter (Millipore).

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Five micrograms of each peptide (AIPLLSPLITSPTSPESDNLKA or KKWQHPASPFCYEPAP, synthesized by Ontores Biotechnology) was incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction (25 m m Tris-HCl, pH 7.5, 1 m m EGTA, 20 m m MgCl 2 , 1 m m DTT, 200 μ m ATP, and 1× phosphatase inhibitor) at room temperature for 1 h. The reaction buffer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9,000 g at 4°C.

    Tandem Mass Spectroscopy:

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Synthetic peptides were incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction, which was then centrifugally filtered through a Millipore 5-kD cutoff filter.

    Article Title: Proteomic and metabolomic analyses of soybean root tips under flooding stress.
    Article Snippet: Flooding is one of the serious problems for soybean plants because it inhibits growth.. Proteomic and metabolomic techniques were used to determine whether proteins and metabolites are altered in the root tips of soybeans under flooding stress.. Two-day-old soybean plants were flooded for 2 days, and proteins and metabolites were extracted from root tips.

    Article Title: Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency
    Article Snippet: The upper layer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9100× g at 4 °C.

    Article Title: Multiple analysis of root exudates and microbiome in rice (Oryza sativa) under low P conditions.
    Article Snippet: Plants release various metabolites from roots and root exudates contribute to differences in stress tolerance among plant species.. Plant and soil microbes have complex interactions that are affected by biotic and abiotic factors.. The purpose of this study was to examine the differences in metabolites in root exudates of rice (Oryza sativa) cultivars and their correlation with bacterial populations in the rhizosphere.

    Article Title: Integrated Analysis of the Effects of Cold and Dehydration on Rice Metabolites, Phytohormones, and Gene Transcripts
    Article Snippet: Metabolites were extracted from aerial parts of rice plants (100 mg) with a chloroform:methanol solution (1:1) using a mixer and a 5-kD cutoff filter (Millipore).

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Five micrograms of each peptide (AIPLLSPLITSPTSPESDNLKA or KKWQHPASPFCYEPAP, synthesized by Ontores Biotechnology) was incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction (25 m m Tris-HCl, pH 7.5, 1 m m EGTA, 20 m m MgCl 2 , 1 m m DTT, 200 μ m ATP, and 1× phosphatase inhibitor) at room temperature for 1 h. The reaction buffer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9,000 g at 4°C.

    In Vitro:

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Synthetic peptides were incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction, which was then centrifugally filtered through a Millipore 5-kD cutoff filter.

    Article Title: Proteomic and metabolomic analyses of soybean root tips under flooding stress.
    Article Snippet: Flooding is one of the serious problems for soybean plants because it inhibits growth.. Proteomic and metabolomic techniques were used to determine whether proteins and metabolites are altered in the root tips of soybeans under flooding stress.. Two-day-old soybean plants were flooded for 2 days, and proteins and metabolites were extracted from root tips.

    Article Title: Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency
    Article Snippet: The upper layer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9100× g at 4 °C.

    Article Title: Multiple analysis of root exudates and microbiome in rice (Oryza sativa) under low P conditions.
    Article Snippet: Plants release various metabolites from roots and root exudates contribute to differences in stress tolerance among plant species.. Plant and soil microbes have complex interactions that are affected by biotic and abiotic factors.. The purpose of this study was to examine the differences in metabolites in root exudates of rice (Oryza sativa) cultivars and their correlation with bacterial populations in the rhizosphere.

    Article Title: Integrated Analysis of the Effects of Cold and Dehydration on Rice Metabolites, Phytohormones, and Gene Transcripts
    Article Snippet: Metabolites were extracted from aerial parts of rice plants (100 mg) with a chloroform:methanol solution (1:1) using a mixer and a 5-kD cutoff filter (Millipore).

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Five micrograms of each peptide (AIPLLSPLITSPTSPESDNLKA or KKWQHPASPFCYEPAP, synthesized by Ontores Biotechnology) was incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction (25 m m Tris-HCl, pH 7.5, 1 m m EGTA, 20 m m MgCl 2 , 1 m m DTT, 200 μ m ATP, and 1× phosphatase inhibitor) at room temperature for 1 h. The reaction buffer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9,000 g at 4°C.

    Purification:

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Synthetic peptides were incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction, which was then centrifugally filtered through a Millipore 5-kD cutoff filter.

    Article Title: Proteomic and metabolomic analyses of soybean root tips under flooding stress.
    Article Snippet: Flooding is one of the serious problems for soybean plants because it inhibits growth.. Proteomic and metabolomic techniques were used to determine whether proteins and metabolites are altered in the root tips of soybeans under flooding stress.. Two-day-old soybean plants were flooded for 2 days, and proteins and metabolites were extracted from root tips.

    Article Title: Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency
    Article Snippet: The upper layer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9100× g at 4 °C.

    Article Title: Multiple analysis of root exudates and microbiome in rice (Oryza sativa) under low P conditions.
    Article Snippet: Plants release various metabolites from roots and root exudates contribute to differences in stress tolerance among plant species.. Plant and soil microbes have complex interactions that are affected by biotic and abiotic factors.. The purpose of this study was to examine the differences in metabolites in root exudates of rice (Oryza sativa) cultivars and their correlation with bacterial populations in the rhizosphere.

    Article Title: Integrated Analysis of the Effects of Cold and Dehydration on Rice Metabolites, Phytohormones, and Gene Transcripts
    Article Snippet: Metabolites were extracted from aerial parts of rice plants (100 mg) with a chloroform:methanol solution (1:1) using a mixer and a 5-kD cutoff filter (Millipore).

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Five micrograms of each peptide (AIPLLSPLITSPTSPESDNLKA or KKWQHPASPFCYEPAP, synthesized by Ontores Biotechnology) was incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction (25 m m Tris-HCl, pH 7.5, 1 m m EGTA, 20 m m MgCl 2 , 1 m m DTT, 200 μ m ATP, and 1× phosphatase inhibitor) at room temperature for 1 h. The reaction buffer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9,000 g at 4°C.

    Mutagenesis:

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Synthetic peptides were incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction, which was then centrifugally filtered through a Millipore 5-kD cutoff filter.

    Article Title: Proteomic and metabolomic analyses of soybean root tips under flooding stress.
    Article Snippet: Flooding is one of the serious problems for soybean plants because it inhibits growth.. Proteomic and metabolomic techniques were used to determine whether proteins and metabolites are altered in the root tips of soybeans under flooding stress.. Two-day-old soybean plants were flooded for 2 days, and proteins and metabolites were extracted from root tips.

    Article Title: Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency
    Article Snippet: The upper layer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9100× g at 4 °C.

    Article Title: Multiple analysis of root exudates and microbiome in rice (Oryza sativa) under low P conditions.
    Article Snippet: Plants release various metabolites from roots and root exudates contribute to differences in stress tolerance among plant species.. Plant and soil microbes have complex interactions that are affected by biotic and abiotic factors.. The purpose of this study was to examine the differences in metabolites in root exudates of rice (Oryza sativa) cultivars and their correlation with bacterial populations in the rhizosphere.

    Article Title: Integrated Analysis of the Effects of Cold and Dehydration on Rice Metabolites, Phytohormones, and Gene Transcripts
    Article Snippet: Metabolites were extracted from aerial parts of rice plants (100 mg) with a chloroform:methanol solution (1:1) using a mixer and a 5-kD cutoff filter (Millipore).

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Five micrograms of each peptide (AIPLLSPLITSPTSPESDNLKA or KKWQHPASPFCYEPAP, synthesized by Ontores Biotechnology) was incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction (25 m m Tris-HCl, pH 7.5, 1 m m EGTA, 20 m m MgCl 2 , 1 m m DTT, 200 μ m ATP, and 1× phosphatase inhibitor) at room temperature for 1 h. The reaction buffer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9,000 g at 4°C.

    Labeling:

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Synthetic peptides were incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction, which was then centrifugally filtered through a Millipore 5-kD cutoff filter.

    Article Title: Proteomic and metabolomic analyses of soybean root tips under flooding stress.
    Article Snippet: Flooding is one of the serious problems for soybean plants because it inhibits growth.. Proteomic and metabolomic techniques were used to determine whether proteins and metabolites are altered in the root tips of soybeans under flooding stress.. Two-day-old soybean plants were flooded for 2 days, and proteins and metabolites were extracted from root tips.

    Article Title: Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency
    Article Snippet: The upper layer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9100× g at 4 °C.

    Article Title: Multiple analysis of root exudates and microbiome in rice (Oryza sativa) under low P conditions.
    Article Snippet: Plants release various metabolites from roots and root exudates contribute to differences in stress tolerance among plant species.. Plant and soil microbes have complex interactions that are affected by biotic and abiotic factors.. The purpose of this study was to examine the differences in metabolites in root exudates of rice (Oryza sativa) cultivars and their correlation with bacterial populations in the rhizosphere.

    Article Title: Integrated Analysis of the Effects of Cold and Dehydration on Rice Metabolites, Phytohormones, and Gene Transcripts
    Article Snippet: Metabolites were extracted from aerial parts of rice plants (100 mg) with a chloroform:methanol solution (1:1) using a mixer and a 5-kD cutoff filter (Millipore).

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Five micrograms of each peptide (AIPLLSPLITSPTSPESDNLKA or KKWQHPASPFCYEPAP, synthesized by Ontores Biotechnology) was incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction (25 m m Tris-HCl, pH 7.5, 1 m m EGTA, 20 m m MgCl 2 , 1 m m DTT, 200 μ m ATP, and 1× phosphatase inhibitor) at room temperature for 1 h. The reaction buffer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9,000 g at 4°C.

    Negative Control:

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Synthetic peptides were incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction, which was then centrifugally filtered through a Millipore 5-kD cutoff filter.

    Article Title: Proteomic and metabolomic analyses of soybean root tips under flooding stress.
    Article Snippet: Flooding is one of the serious problems for soybean plants because it inhibits growth.. Proteomic and metabolomic techniques were used to determine whether proteins and metabolites are altered in the root tips of soybeans under flooding stress.. Two-day-old soybean plants were flooded for 2 days, and proteins and metabolites were extracted from root tips.

    Article Title: Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency
    Article Snippet: The upper layer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9100× g at 4 °C.

    Article Title: Multiple analysis of root exudates and microbiome in rice (Oryza sativa) under low P conditions.
    Article Snippet: Plants release various metabolites from roots and root exudates contribute to differences in stress tolerance among plant species.. Plant and soil microbes have complex interactions that are affected by biotic and abiotic factors.. The purpose of this study was to examine the differences in metabolites in root exudates of rice (Oryza sativa) cultivars and their correlation with bacterial populations in the rhizosphere.

    Article Title: Integrated Analysis of the Effects of Cold and Dehydration on Rice Metabolites, Phytohormones, and Gene Transcripts
    Article Snippet: Metabolites were extracted from aerial parts of rice plants (100 mg) with a chloroform:methanol solution (1:1) using a mixer and a 5-kD cutoff filter (Millipore).

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Five micrograms of each peptide (AIPLLSPLITSPTSPESDNLKA or KKWQHPASPFCYEPAP, synthesized by Ontores Biotechnology) was incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction (25 m m Tris-HCl, pH 7.5, 1 m m EGTA, 20 m m MgCl 2 , 1 m m DTT, 200 μ m ATP, and 1× phosphatase inhibitor) at room temperature for 1 h. The reaction buffer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9,000 g at 4°C.

    Nucleic Acid Electrophoresis:

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Synthetic peptides were incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction, which was then centrifugally filtered through a Millipore 5-kD cutoff filter.

    Article Title: Proteomic and metabolomic analyses of soybean root tips under flooding stress.
    Article Snippet: Flooding is one of the serious problems for soybean plants because it inhibits growth.. Proteomic and metabolomic techniques were used to determine whether proteins and metabolites are altered in the root tips of soybeans under flooding stress.. Two-day-old soybean plants were flooded for 2 days, and proteins and metabolites were extracted from root tips.

    Article Title: Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency
    Article Snippet: The upper layer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9100× g at 4 °C.

    Article Title: Multiple analysis of root exudates and microbiome in rice (Oryza sativa) under low P conditions.
    Article Snippet: Plants release various metabolites from roots and root exudates contribute to differences in stress tolerance among plant species.. Plant and soil microbes have complex interactions that are affected by biotic and abiotic factors.. The purpose of this study was to examine the differences in metabolites in root exudates of rice (Oryza sativa) cultivars and their correlation with bacterial populations in the rhizosphere.

    Article Title: Integrated Analysis of the Effects of Cold and Dehydration on Rice Metabolites, Phytohormones, and Gene Transcripts
    Article Snippet: Metabolites were extracted from aerial parts of rice plants (100 mg) with a chloroform:methanol solution (1:1) using a mixer and a 5-kD cutoff filter (Millipore).

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Five micrograms of each peptide (AIPLLSPLITSPTSPESDNLKA or KKWQHPASPFCYEPAP, synthesized by Ontores Biotechnology) was incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction (25 m m Tris-HCl, pH 7.5, 1 m m EGTA, 20 m m MgCl 2 , 1 m m DTT, 200 μ m ATP, and 1× phosphatase inhibitor) at room temperature for 1 h. The reaction buffer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9,000 g at 4°C.

    Synthesized:

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Synthetic peptides were incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction, which was then centrifugally filtered through a Millipore 5-kD cutoff filter.

    Article Title: Proteomic and metabolomic analyses of soybean root tips under flooding stress.
    Article Snippet: Flooding is one of the serious problems for soybean plants because it inhibits growth.. Proteomic and metabolomic techniques were used to determine whether proteins and metabolites are altered in the root tips of soybeans under flooding stress.. Two-day-old soybean plants were flooded for 2 days, and proteins and metabolites were extracted from root tips.

    Article Title: Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency
    Article Snippet: The upper layer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9100× g at 4 °C.

    Article Title: Multiple analysis of root exudates and microbiome in rice (Oryza sativa) under low P conditions.
    Article Snippet: Plants release various metabolites from roots and root exudates contribute to differences in stress tolerance among plant species.. Plant and soil microbes have complex interactions that are affected by biotic and abiotic factors.. The purpose of this study was to examine the differences in metabolites in root exudates of rice (Oryza sativa) cultivars and their correlation with bacterial populations in the rhizosphere.

    Article Title: Integrated Analysis of the Effects of Cold and Dehydration on Rice Metabolites, Phytohormones, and Gene Transcripts
    Article Snippet: Metabolites were extracted from aerial parts of rice plants (100 mg) with a chloroform:methanol solution (1:1) using a mixer and a 5-kD cutoff filter (Millipore).

    Article Title: The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response
    Article Snippet: Five micrograms of each peptide (AIPLLSPLITSPTSPESDNLKA or KKWQHPASPFCYEPAP, synthesized by Ontores Biotechnology) was incubated with His-SlMPK1 and GST-SlMKK2 DD in a kinase reaction (25 m m Tris-HCl, pH 7.5, 1 m m EGTA, 20 m m MgCl 2 , 1 m m DTT, 200 μ m ATP, and 1× phosphatase inhibitor) at room temperature for 1 h. The reaction buffer was centrifugally filtered through a Millipore 5-kD cutoff filter at 9,000 g at 4°C.



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    Average 90 stars, based on 1 article reviews
    3‐kd cutoff filters (amicon ultra‐5 - by Bioz Stars, 2026-09
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    Harvard Bioscience 5 kd molecular weight cutoff dispodialyzer filter
    5 Kd Molecular Weight Cutoff Dispodialyzer Filter, supplied by Harvard Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5-kd+cutoff+filter/5+kd+molecular+weight+cutoff+dispodialyzer+filter/pmc06800125-92-7-13
    Average 90 stars, based on 1 article reviews
    5 kd molecular weight cutoff dispodialyzer filter - by Bioz Stars, 2026-09
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